t4 dna ligase (New England Biolabs)
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New England Biolabs
t4 dna ligase
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+ligase/T4+DNA+Ligase/pm42894212-57-40-44
Average 99 stars, based on 33799 article reviews
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+ligase/T4+DNA+Ligase/pm42894212-57-40-44
Average 99 stars, based on 33799 article reviews
t4 dna ligase - by Bioz Stars,
2026-10
99/100 stars
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Bioprocessing:Article Title: Targeted Insertion of Partial CFTR cDNA Replicates Native Expression Pattern with Restored Channel Function in CF Airway Epithelium Article Snippet: Each element was 574 subcloned through In-Fusion (#638952, Takara, San Jose, CA, USA). .. The final donor was subcloned into 575 AAV-2 ITR-containing plasmid backbone (pAAV2-MCS from VPK-410, Cell Biolabs, San Diego, CA, 576 USA) or HIV LTR;cPPT/CTS;RRE;HIV-1-Ѱ-containing backbone (#78852, Addgene) through enzyme 577 ligation with Plasmid Preparation:Article Title: Targeted Insertion of Partial CFTR cDNA Replicates Native Expression Pattern with Restored Channel Function in CF Airway Epithelium Article Snippet: Each element was 574 subcloned through In-Fusion (#638952, Takara, San Jose, CA, USA). .. The final donor was subcloned into 575 AAV-2 ITR-containing plasmid backbone (pAAV2-MCS from VPK-410, Cell Biolabs, San Diego, CA, 576 USA) or HIV LTR;cPPT/CTS;RRE;HIV-1-Ѱ-containing backbone (#78852, Addgene) through enzyme 577 ligation with Article Title: Geometry of braided DNA dictates supercoiling partitioning. Article Snippet: This intermediate plasmid had an unwanted Nt.BbvCI (NEB, R0632) recognition sequence on top of four that was included by design. .. This site was removed using site directed mutagenesis, first using PCR amplification from the intermediate plasmid with primers (KLD nickRemoval F, KLD nickRemoval R, Supplementary Table 4), followed by a KLD reaction on the PCR product with T4 PNK (NEB, M0201S), Article Title: A Salmonella phage steers bacterial populations toward hypersensitivity to benzalkonium chloride, hydrogen peroxide, and triclosan. Article Snippet: .. Deletion constructs for wcaJ and rfaB were generated by overlap extension PCR as described previously (45), and cloned into the suicide vector pRE112 (Addgene, Watertown, MA, USA) using Ligation:Article Title: Targeted Insertion of Partial CFTR cDNA Replicates Native Expression Pattern with Restored Channel Function in CF Airway Epithelium Article Snippet: Each element was 574 subcloned through In-Fusion (#638952, Takara, San Jose, CA, USA). .. The final donor was subcloned into 575 AAV-2 ITR-containing plasmid backbone (pAAV2-MCS from VPK-410, Cell Biolabs, San Diego, CA, 576 USA) or HIV LTR;cPPT/CTS;RRE;HIV-1-Ѱ-containing backbone (#78852, Addgene) through enzyme 577 ligation with Cloning:Article Title: Suppression of Loss of the FabH 3-Ketoacyl-ACP Synthase III by a KAS II (FabF) Lacking the Active Site Thiol in Enterococcus faecalis. Article Snippet: Anti-DYKDDDDK G1 Affinity Resin was purchased from GeneScript and the FLAG tag was purchased from MedChemExpress. .. DNA polymerase, restriction endonuclease, Lambda DNA Preparation:Article Title: Single-molecule imaging reveals DNA shape read-out by the INO80 chromatin remodeler Article Snippet: .. Oligos containing biotin (5'-P-AGGTCGCCGCCC-bio-3') or digoxigenin (5'-P-GGGCGGCGACCT-dig-3') were hybridized to the cos sites of the lambda DNA, ligated overnight at room temperature using Purification:Article Title: Single-molecule imaging reveals DNA shape read-out by the INO80 chromatin remodeler Article Snippet: .. Oligos containing biotin (5'-P-AGGTCGCCGCCC-bio-3') or digoxigenin (5'-P-GGGCGGCGACCT-dig-3') were hybridized to the cos sites of the lambda DNA, ligated overnight at room temperature using Mutagenesis:Article Title: Geometry of braided DNA dictates supercoiling partitioning. Article Snippet: This intermediate plasmid had an unwanted Nt.BbvCI (NEB, R0632) recognition sequence on top of four that was included by design. .. This site was removed using site directed mutagenesis, first using PCR amplification from the intermediate plasmid with primers (KLD nickRemoval F, KLD nickRemoval R, Supplementary Table 4), followed by a KLD reaction on the PCR product with T4 PNK (NEB, M0201S), Polymerase Chain Reaction:Article Title: Geometry of braided DNA dictates supercoiling partitioning. Article Snippet: This intermediate plasmid had an unwanted Nt.BbvCI (NEB, R0632) recognition sequence on top of four that was included by design. .. This site was removed using site directed mutagenesis, first using PCR amplification from the intermediate plasmid with primers (KLD nickRemoval F, KLD nickRemoval R, Supplementary Table 4), followed by a KLD reaction on the PCR product with T4 PNK (NEB, M0201S), Article Title: A Salmonella phage steers bacterial populations toward hypersensitivity to benzalkonium chloride, hydrogen peroxide, and triclosan. Article Snippet: .. Deletion constructs for wcaJ and rfaB were generated by overlap extension PCR as described previously (45), and cloned into the suicide vector pRE112 (Addgene, Watertown, MA, USA) using Amplification:Article Title: Geometry of braided DNA dictates supercoiling partitioning. Article Snippet: This intermediate plasmid had an unwanted Nt.BbvCI (NEB, R0632) recognition sequence on top of four that was included by design. .. This site was removed using site directed mutagenesis, first using PCR amplification from the intermediate plasmid with primers (KLD nickRemoval F, KLD nickRemoval R, Supplementary Table 4), followed by a KLD reaction on the PCR product with T4 PNK (NEB, M0201S), Construct:Article Title: A Salmonella phage steers bacterial populations toward hypersensitivity to benzalkonium chloride, hydrogen peroxide, and triclosan. Article Snippet: .. Deletion constructs for wcaJ and rfaB were generated by overlap extension PCR as described previously (45), and cloned into the suicide vector pRE112 (Addgene, Watertown, MA, USA) using Generated:Article Title: A Salmonella phage steers bacterial populations toward hypersensitivity to benzalkonium chloride, hydrogen peroxide, and triclosan. Article Snippet: .. Deletion constructs for wcaJ and rfaB were generated by overlap extension PCR as described previously (45), and cloned into the suicide vector pRE112 (Addgene, Watertown, MA, USA) using Clone Assay:Article Title: A Salmonella phage steers bacterial populations toward hypersensitivity to benzalkonium chloride, hydrogen peroxide, and triclosan. Article Snippet: .. Deletion constructs for wcaJ and rfaB were generated by overlap extension PCR as described previously (45), and cloned into the suicide vector pRE112 (Addgene, Watertown, MA, USA) using Incubation:Article Title: A Salmonella phage steers bacterial populations toward hypersensitivity to benzalkonium chloride, hydrogen peroxide, and triclosan. Article Snippet: .. Deletion constructs for wcaJ and rfaB were generated by overlap extension PCR as described previously (45), and cloned into the suicide vector pRE112 (Addgene, Watertown, MA, USA) using |
