t4 dna ligase (New England Biolabs)
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New England Biolabs
t4 dna ligase
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+ligase/T4+DNA+Ligase/pmc12996642-80-33-36
Average 99 stars, based on 33541 article reviews
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+ligase/T4+DNA+Ligase/pmc12996642-80-33-36
Average 99 stars, based on 33541 article reviews
t4 dna ligase - by Bioz Stars,
2026-09
99/100 stars
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Cloning:Article Title: A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation. Article Snippet: .. For cloning linear gene fragments into KanR1_receiver (pCM001), KanR2_individual_validation_receiver (pCM023), and KanR_monovalent_receiver (pCM025), AR TI CL E IN P RE SS 0.25 μL of 60 ng/μL landing pad plasmids, 2.5 μL of 20 nM gene fragment, 0.5 μL of Plasmid Preparation:Article Title: PARP4 ADP-ribosylates PIDD1 to complete a phospho/SUMO/PAR-ylation cascade that orchestrates PIDDosome assembly Article Snippet: .. Hybridized oligonucleotides were diluted 1:200 with H 2 O. BsmBI-v2–digested lentiCRISPR v2 plasmid (50 ng) was ligated with 1 μM final concentration oligo duplex using Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Article Snippet: 4 of 24 1 fused to Maroon1 from pLL3.7m-mTurquoise2-SLBP was eliminated by excision of the vector with EcoRI-HF (NEB, R3101S). .. After gel purification using the QIAquick Gel Extraction Kit (Qiagen, 28706), the resulting cut vector was religated overnight with Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Article Snippet: The cut vectors were gel-purified using the IAquick Gel Extraction Kit (Qiagen, 28706). .. The PCR products ere cloned into each lentiviral vector overnight by classical igation with Concentration Assay:Article Title: PARP4 ADP-ribosylates PIDD1 to complete a phospho/SUMO/PAR-ylation cascade that orchestrates PIDDosome assembly Article Snippet: .. Hybridized oligonucleotides were diluted 1:200 with H 2 O. BsmBI-v2–digested lentiCRISPR v2 plasmid (50 ng) was ligated with 1 μM final concentration oligo duplex using Incubation:Article Title: PARP4 ADP-ribosylates PIDD1 to complete a phospho/SUMO/PAR-ylation cascade that orchestrates PIDDosome assembly Article Snippet: .. Hybridized oligonucleotides were diluted 1:200 with H 2 O. BsmBI-v2–digested lentiCRISPR v2 plasmid (50 ng) was ligated with 1 μM final concentration oligo duplex using Article Title: A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation. Article Snippet: Each effector was ordered from Twist Biosciences as AR TI CL E IN P RE SS a clonal gene in pTwist Kan High Copy with flanking sequences containing nested BsmBI and BsaI golden gate sites to facilitate cloning bivalent combinations (Supplementary Fig. 3a and Supplementary Data 9, Misc., 5′ and 3′ Effector Flank). .. The BsmBI sites in this pool (EF2 pool) were later used to clone into the N-terminal Effector 2 position, and the BsaI sites were used to subclone the pooled effectors into a new high copy Chloramphenicol resistant backbone via golden gate reaction (50 ng pMH249 (Supplementary Data 9, Plasmids), 2-fold molar excess EF2 pool, 1 μL BsaI-HFv2 (NEB, R3733L), 1 μL Gel Purification:Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Article Snippet: 4 of 24 1 fused to Maroon1 from pLL3.7m-mTurquoise2-SLBP was eliminated by excision of the vector with EcoRI-HF (NEB, R3101S). .. After gel purification using the QIAquick Gel Extraction Kit (Qiagen, 28706), the resulting cut vector was religated overnight with Gel Extraction:Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Article Snippet: 4 of 24 1 fused to Maroon1 from pLL3.7m-mTurquoise2-SLBP was eliminated by excision of the vector with EcoRI-HF (NEB, R3101S). .. After gel purification using the QIAquick Gel Extraction Kit (Qiagen, 28706), the resulting cut vector was religated overnight with other:Article Title: Deep mutational scan of the pore of the cold-sensing TRPM8 channel Article Snippet: The specificity of the reaction was verified by agarose gel electrophoresis, and the amplified oligos were purified using the Oligo Clean & Concentrator kit (Zymo Research) and an elution volume of 15 μL of 60 °C nuclease-free water, and their concentration after purification was measured on a fluorescent plate reader using PicoGreen reagent (Invitrogen) and 96-well plates. Polymerase Chain Reaction:Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Article Snippet: The cut vectors were gel-purified using the IAquick Gel Extraction Kit (Qiagen, 28706). .. The PCR products ere cloned into each lentiviral vector overnight by classical igation with Clone Assay:Article Title: Cell Cycle Control of Nuclear Metabolism Couples Phosphatidylinositol Signaling to Histone Methylation. Article Snippet: The cut vectors were gel-purified using the IAquick Gel Extraction Kit (Qiagen, 28706). .. The PCR products ere cloned into each lentiviral vector overnight by classical igation with Ligation:Article Title: A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation. Article Snippet: Each effector was ordered from Twist Biosciences as AR TI CL E IN P RE SS a clonal gene in pTwist Kan High Copy with flanking sequences containing nested BsmBI and BsaI golden gate sites to facilitate cloning bivalent combinations (Supplementary Fig. 3a and Supplementary Data 9, Misc., 5′ and 3′ Effector Flank). .. The BsmBI sites in this pool (EF2 pool) were later used to clone into the N-terminal Effector 2 position, and the BsaI sites were used to subclone the pooled effectors into a new high copy Chloramphenicol resistant backbone via golden gate reaction (50 ng pMH249 (Supplementary Data 9, Plasmids), 2-fold molar excess EF2 pool, 1 μL BsaI-HFv2 (NEB, R3733L), 1 μL |